Their contents were higher in the V-shaped canopy than in the T-shaped and VSP canopies. A total of 96 flavonoids and 32 phenolic acids were detected among the DRMs. Transcriptome and metabolome analyses revealed that the differentially expressed genes (DEGs) and differentially regulated metabolites (DRMs) were significantly enriched in the flavonoid and phenylpropanoid biosynthesis pathways. Results showed that the V-shaped canopy was more favorable for the accumulation of flavonoids and phenolic acids. The flavonoids and phenolic acids in the berry pericarps from grapevines pruned using three types of leaf canopy, namely, V-shaped, T-shaped, and vertical shoot-positioned (VSP) canopies, were compared in this study. Various methods are used to shape and prune grapevines, but their effects on the flavonoids and phenolic acids remain unclear. The flavonoids and phenolic acids in grape berries greatly influence the quality of wine.
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